Next Generation Sequencing
DNA sequencing is a vital part of Fera's research on crops, food and diseases. We routinely use Illumina sequencing technology for our work on pathogen genomics, disease diagnosis and microbial community analysis. Our team of sequencing technicians operate an Illumina Miseq instrument, backed up by powerful sequence processing hardware and a team of bioinformaticians.
One of the major uses of our next generation sequencing technology is non-targeted analysis of biological components in a sample for diagnostic and detection purposes from a range of sample material of plant, animal and food origin. Fera has successfully used these novel technologies for detection of previously unknown pathogens and is using it for microbial community profiling in a multitude of research projects. Novel developments in DNA sequencing technology have delivered an increase in achievable read length of DNA sequences which is a prerequisite for reliable species identification. Furthermore, bacterial genomics with focus on plant and foodborne pathogens forms an integral part of our sequencing portfolio.
Fera offers high throughput DNA sequencing both as a collaborative partner but also as a service. Please send enquiries to firstname.lastname@example.org
Some of the work that includes our DNA sequencing capability is listed below:
- Adams, I.P. et al. 2009. Next-generation sequencing and metagenomic analysis: a universal diagnostic tool in plant virology. Molecular Plant Pathology 10(4) 537-45.
- Green, S., Studholme, D., Laue, B., Dorati, F., Lovell, H., Arnold, D., Cottrell, J., Bridgett, S., Blaxter, M., Huitema, E., Thwaites, R., Sharp, P., Jackson, R., Kamoun, S. 2010. Comparative genome analysis provides insights into the evolution and adaptation of Pseudomonas syringae pv. aesculi on Aesculus hippocastanum. PLoS ONE 5(4): e10224. doi:10.1371/journal.pone.0010224
- Studholme, DJ., Kemen, E., MacLean, D., Schornack, S., Aritua, V., Thwaites, R., Grant, M., Smith, J. and Jones, JDG. 2010. Genome-wide sequencing data reveals virulence factors implicated in banana Xanthomonas wilt. FEMS Microbiology Letters 310(2) 182-92.
- Monger, W.A., Adams, I.P., Glover, R.H., Barrett, B. 2010. The complete genome sequence of Canna yellow streak virus.Arch Virol 155(9):1515-8.
- Wendy A Monger, T Alicai, J Ndunguru, Z M Kinyua, M Potts, R H Reeder, D W Miano, I P Adams, N Boonham, R H Glover, J Smith (2010) The complete genome sequence of the Tanzanian strain of Cassava brown streak virus and comparison with the Ugandan strain sequence. Arch Virol 155(3):429-433.
- Studholme, DJ., Wasukira, A., Paszkiewicz, K., Aritua, V., Thwaites, R., Smith, J. and Grant, M. 2011. Draft genome sequences of Xanthomonas sacchari and two banana-associated Xanthomonads reveal insights into the Xanthomonas group 1 clade. Genes 2 1050-1065; doi: 10.3390/genes2041050.
- Adams, I. P., Miano, D. W., Kinyua, Z. M., Wangai, A., Kimani, E., Phiri, N., Reeder, R., Harju, V., Glover, R., Hany, U., Souza-Richards, R., Deb Nath, P., Nixon, T., Fox, A., Barnes, A., Smith, J., Skelton, A., Thwaites, R., Mumford, R., Boonham, N. 2012. Use of next-generation sequencing for the identification and characterization of Maize chlorotic mottle virus and Sugarcane mosaic virus causing maize lethal necrosis in Kenya. Plant Pathology DOI:10.1111/j.1365-3059.2012.0690.x.
Current and recent projects